Analysing endometrial microbiome: methodological considerations and recommendations for good practice

ABSTRACT There is growing evidence that the upper female genital tract not sterile, harbouring its own microbial communities. However, significance and potential effect of endometrial microorganisms on reproductive functions remain to be fully elucidated. Analysing microbiome, microbes their genetic material present in endometrium, an emerging area study. The initial studies suggest it associated with poor outcomes different gynaecological pathologies. Nevertheless, studying a low-biomass niche as challenge conduct well-designed well-controlled experiments order avoid adjust for risk contamination, especially from lower tract. Herein, we aim highlight methodological considerations propose good practice recommendations future microbiome studies.